Phylogenetic footprinting online dating Linz dating
Therefore in silico evidences between TFs and TFBSs can be valuable.
In microalgae, however, computational identifications of transcriptional factors (TFs) genome-wide were reported only for green algae such as Chlamydomonas reinhardtii and Volvox carteri.
are a group of oleaginous microalgae that harbor an expanded array of lipid-synthesis related genes, yet how they are transcriptionally regulated remains unknown.
Here a phylogenomic approach was employed to identify and functionally annotate the transcriptional factors (TFs) and TF binding-sites (TFBSs) in N. Among 36 microalgae and higher plants genomes, a two-fold reduction in the number of TF families plus a seven-fold decrease of average family-size in Nannochloropsis, Rhodophyta and Chlorophyta were observed.
In the three strains, 26 TF families were found collectively, among which 19 are shared by all three strains.
The degree of similarity in TF-family profiles is indicative of the phylogenetic relationship among the species, suggesting co-evolution of TF-family profiles and species.
Furthermore, comparative analysis of six Nannochloropsis genomes revealed 68 “most-conserved” TFBS motifs, with 11 of which predicted to be related to lipid accumulation or photosynthesis.
In higher plants, most MYB genes encode proteins of the R2R3-MYB class (e.g., Arabidopsis thaliana harbors 131 R2R3-MYB genes yet only five R1R2R3-MYB genes.
Intriguingly, the proportions of R1R2R3-MYB genes are significantly higher in the three Nannochloropsis strains (e.g.